Authors should fully disclose all posting in registries of results of the same or closely related work. Tables can be placed either next to the relevant text in the article, or on separate page(s) at the end. 4). Corresponding authors will receive an e-mail with a link to our online proofing system, allowing annotation and correction of proofs online. Sinclair R., Flagler M., Jones L., Rufaut N., Davis M. The proteomic profile of hair damage. Use a logical naming convention for your artwork files. New consensus nomenclature for mammalian keratins. Any further information, if known (DOI, author names, dates, reference to a source publication, etc. SDS PAGE analysis of proteins in E. coli cells expressing keratin K31. Reporting clinical trials Randomized controlled trials should be presented according to the CONSORT guidelines. Short Communications: These should be no more than 2,500 words, with up to 20 references and a maximum of 3 figures or tables. In particular, do not use the options to justify text or to hyphenate words. Present simple formulae in line with normal text where possible and use the solidus (/) instead of a horizontal line for small fractional terms, e.g., X/Y. The human keratin K31 being a major constituent of human hair and having high level of similarity with the rest of the human hair keratin types [34,35], would make it more effective in hair improvement. Manuscripts Please type all pages with double spacing and wide margins on one side of the paper. When preparing tables, if you are using a table grid, use only one grid for each individual table and not a grid for each row. If you agree, your manuscript will be transferred, though you will have the opportunity to make changes to the manuscript before the submission is complete. The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online Open Access journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides . The functionality is limited to basic scrolling. Langbein L., Rogers M.A., Praetzel-Wunder S., Bckler D., Schirmacher P., Schweizer J. Similarly, the force required to break the hair before and after the chemical treatment was 20N and 11N, respectively. Elsevier remains neutral with regard to any jurisdictional claims. molecular epidemiology of resistant organisms; drug-resistant viral emergence in treated and untreated patients; microbiological and clinical studies stewardship; antimicrobial resistance testing detection and diagnostic; viral resistant variants and impact on public health; PK/PD parameters and prediction of efficacy; surveillance studies of resistant pathogens affecting humans isolated from different hosts and environments. They consist of a short collection of bullet points that capture the novel results of your research as well as new methods that were used during the study (if any). Purely observational studies (those in which the assignment of the medical intervention is not at the discretion of the investigator) will not require registration. Keep text in the illustrations themselves to a minimum but explain all symbols and abbreviations used. Commercially used keratins are isolated from wool or chicken feathers. Online proof correction To ensure a fast publication process of the article, we kindly ask authors to provide us with their proof corrections within two days. CD5 molecule-like and transthyretin as putative biomarkers of chronic myeloid leukemia-an insight from the proteomic analysis of human plasma. Non-standard or uncommon abbreviations should be avoided in the abstract, but if essential they must be defined at their first mention in the abstract itself. Sitemap. The clinical trial registration number should be included at the end of the abstract of the article. Please supply 'stills' with your files: you can choose any frame from the video or animation or make a separate image. Significant changes to the article as accepted for publication will only be considered at this stage with permission from the Editor. The bound protein was eluted with a continuous NaCl gradient (0.11M) prepared in Tris buffer at a flow rate of 1ml per minute. Authorship All authors should have made substantial contributions to all of the following: (1) the conception and design of the study, or acquisition of data, or analysis and interpretation of data, (2) drafting the article or revising it critically for important intellectual content, (3) final approval of the version to be submitted. Sign in to view your account details and order history, Journal of Global Antimicrobial Resistance, 81 editors and editorial board members in 29 countries/regions, Gender Diversity Distribution of the Editors. Any addition, deletion or rearrangement of author names in the authorship list should be made only before the manuscript has been accepted and only if approved by the journal Editor. If the manuscript has already been published in an online issue, any requests approved by the Editor will result in a corrigendum. Enhanced expression and activity yields of Clostridium thermocellum xylanases without non-catalytic domains. Provide captions to illustrations separately. If you wish to make changes to supplementary material during any stage of the process, please make sure to provide an updated file. To determine the straightening ability of K31, 6080 hairs from a curly tress of average 10cm length was placed in 100ml of 0.75M NaOH for 30min in orbital shaker at 120rpm. Both the naturally damaged hair and those partially damaged by bleach treatment showed gain in their diameter after keratin treatment. Be sparing in the use of tables and ensure that the data presented in them do not duplicate results described elsewhere in the article. Data statement To foster transparency, we encourage you to state the availability of your data in your submission. The preprint DOI should also be provided. The recombinant cloning vector pEX-K was double digested with NdeI and EcoRI and ligated into linearized pET-22b (+) using T4 DNA ligase followed by transformation initially into E. coli DH5 cells using the standard protocol [16]. Access Elseviers most recent Global Gender Report. The cystine content of hair and other epidermal tissues. Studier F.W. Increased smoothness of the hair surfaces should make a significant contribution in not only improving their look but also in eliminating entanglement, if any, more easily. The hairs were then rinsed three times with water and air-dried. For supported data repositories a repository banner will automatically appear next to your published article on ScienceDirect. Keratin K31, the major component of the hair structure, has an important role in maintenance of hair in a healthy state. An abstract is often presented separately from the article, so it must be able to stand alone. We advise to seek gender neutrality by using plural nouns ("clinicians, patients/clients") as default/wherever possible to avoid using "he, she," or "he/she." Application of K31 resulted in marked improvements in smoothness, diameter and mechanical strength of the damaged hair. The authors have declared no conflict of interest. As the medium have both glucose and lactose as carbon sources, E. coli shall utilize glucose first through carbon catabolite repression mechanism until it is exhausted followed by utilization of lactose as the carbon source [26]. Mean values of the stress (the force required to break the hair) before and after keratin treatment are given in Table 1. Highlights Highlights are mandatory for this journal as they help increase the discoverability of your article via search engines. The terms sex and gender should be used correctly. Preferred file types: TIFF, EPS, PDF or MS Office files. For statistical analysis the standard error and t-test (P0.05) was used to differentiate the mean effect of treatments on the studied parameters (hair thickness, Youngs modulus and breaking force). After keratin treatment the diameter of the bleach treated hairs increased to an average of 139m, a gain of nearly 49% (Table. National Cheng Kung University Department of Medical Laboratory Science and Biotechnology, Tainan, Taiwan. Elsevier remains neutral with regard to any jurisdictional claims. All instructions for proofing will be given in the e-mail we send to authors, including alternative methods to the online version and PDF.We will do everything possible to get your article published quickly and accurately. Research data This journal encourages and enables you to share data that supports your research publication where appropriate, and enables you to interlink the data with your published articles. However, the intact protein has important implications in restoring or improving the mechanical strength of the damaged hair fibers [13,14]. See also the section on Electronic artwork. However, the intact protein in its native state can be more effective in forming a cohesive film by filling the gaps produced in the damaged hair [24]. When preparing tables, if you are using a table grid, use only one grid for each individual table and not a grid for each row. Methods that are already published should be summarized, and indicated by a reference. Health improvement of human hair and their reshaping using recombinant keratin K31. The objective of this study was to produce recombinant K31 in a native form and study its role in the treatment of naturally and chemically damaged hairs. Footnotes Footnotes should be used sparingly. Five fibers from each of the hair tress were randomly selected and incubated in 15ml 0.05M Tris-Cl buffer (pH 7.5) containing 0.2mgml1 K31protein in a petri plate for 1h in an orbital shaker at 120rpm. Preprints should be clearly marked as such, for example by including the word preprint, or the name of the preprint server, as part of the reference. Please avoid using vertical rules and shading in table cells. These guidelines are meant as a point of reference to help identify appropriate language but are by no means exhaustive or definitive. The ePub format uses eBook readers, which have several "ease of reading" features Schweizer J., Bowden P.E., Coulombe P.A., Langbein L., Lane E.B., Magin T.M., Maltais L., Omary M.B., Parry D.A.D., Rogers M.A., Wright M.W. Data references This journal encourages you to cite underlying or relevant datasets in your manuscript by citing them in your text and including a data reference in your Reference List. The ability of K31 to straighten the curly hair and repairing the chemically damaged hair was investigated. Tensile strength of the naturally damaged and the bleach-treated hair showed marked improvement after keratin treatment. At manuscript submission, authors must provide the CONSORT checklist accompanied by a flow diagram that illustrates the progress of patients through the trial, including recruitment, enrollment, randomization, withdrawal and completion, and a detailed description of the randomization procedure. Italics are not to be used for expressions of Latin origin, for example, in vivo, et al., per se. AA361117.Authors are encouraged to check accession numbers used very carefully. aSchool of Biological Sciences, University of the Punjab, Lahore, Pakistan, bInstitute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan. Editorial Review All manuscripts are subject to peer review. Human hair cuticle: biologically conspired to the owners advantage. Use this numbering also for internal cross-referencing: do not just refer to 'the text'. 1). The proteomics of keratin proteins. Number tables consecutively in accordance with their appearance in the text and place any table notes below the table body. If your computer features the option to save files 'in flat ASCII', please do not use it.Please write your text in good English (American or British usage is accepted, but not a mixture of these). Registration of clinical trials Registration in a public trials registry is a condition for publication of clinical trials in this journal in accordance with International Committee of Medical Journal Editors recommendations. This means that if an editor feels your manuscript is more suitable for an alternative journal, you might be asked to consider transferring the manuscript to such a journal. Nakamura A., Arimoto M., Takeuchi K., Fujii T. A rapid extraction procedure of human hair proteins and identification of phosphorylated species. Aberdeen Royal Infirmary, Aberdeen, United Kingdom, Queen Mary Hospital, Hong Kong, Hong Kong, China Medical University Hospital Department of Internal Medicine, Taichung, Taiwan, University of Catania Department of Biomedical and Biotechnological Sciences, Catania, Italy, Resistance Mechanisms and Gene Transfer, Resistome and Proteome, Case Western Reserve University, Cleveland, Ohio, United States of America, Beta-lactamases, beta-lactam antibiotics, antimicrobial resistance, Smolensk State Medical Universit National Research Institute of Antimicrobial Chemotherapy, Smolensk, Russian Federation, Microbiology,Antimicrobial Resistance,Molecular Epidemiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, National and Kapodistrian University of Athens, Athens, Greece, Molecular Epidemiology of Resistant Organisms, Kaohsiung Medical University, Internal medicine, Kaohsiung, Taiwan, University Institute of Health Sciences, Gandra, Portugal, Antimicrobial Resistance,Molecular epidemiology of bacterial pathogens,Forensics Microbiology,One Health,Genomics,Infectious Diseases, Microbiological and Clinical Studies Stewardship, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy, University of Rome La Sapienza, Roma, Italy, University General Hospital Athinon, Athens, Greece, National Cheng Kung University Hospital, Div. 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